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bone marrow stroma cell bmsc line hs 5  (ATCC)


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    Structured Review

    ATCC bone marrow stroma cell bmsc line hs 5
    DCZ0814 induced cytotoxicity in MM cells and overcome the protective effect of the BM microenvironment. ( A ) Molecular structure of DCZ0814. ( B ) MM cells treated with DCZ0814 for 48 h and cell viability determined by CCK-8 assay. ( C ) MM cells treated with DCZ0814 for the indicated time and cell viability determined by CCK-8 assay. ( D and E ) Cell viability of ARP1 and OCI-MY5 cells treated with DCZ0814 alone or in the presence of IL-6 (25 ng/mL), IGF-1 (25 ng/mL) or BMSCs (HS-5) for 48 h. * p <0.05 compared with the 0 μM group, & p <0.05. ( F ) The percentage of Annexin-V positive cells. * p <0.05 compared with the 0 μM group. ( G ) BMSCs, ARP1, or both ARP1 and BMSCs cells were treated with DCZ0814 for 48 h, and apoptosis was detected by Annexin-V/PI staining followed by flow cytometry. Green represents BMSCs HS-5 cells, and red represents ARP1 cells. In each case, data are presented as the mean ± SD of triplicate treatments. Abbreviations: MM, multiple myeoma; BM, bone marrow; CCK-8, cell counting kit-8; IL-6, interleukin-6; IGF-1, insulin-like growth factor-1; <t>BMSC,</t> bone marrow stromal cell.
    Bone Marrow Stroma Cell Bmsc Line Hs 5, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bone+marrow+stroma+cell+bmsc+line+hs+5/HS-5/pmc06549748-13-5-16
    Average 97 stars, based on 333 article reviews
    bone marrow stroma cell bmsc line hs 5 - by Bioz Stars, 2026-10
    97/100 stars

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    1) Product Images from "DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2"

    Article Title: DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2

    Journal: Cancer Management and Research

    doi: 10.2147/CMAR.S194202

    DCZ0814 induced cytotoxicity in MM cells and overcome the protective effect of the BM microenvironment. ( A ) Molecular structure of DCZ0814. ( B ) MM cells treated with DCZ0814 for 48 h and cell viability determined by CCK-8 assay. ( C ) MM cells treated with DCZ0814 for the indicated time and cell viability determined by CCK-8 assay. ( D and E ) Cell viability of ARP1 and OCI-MY5 cells treated with DCZ0814 alone or in the presence of IL-6 (25 ng/mL), IGF-1 (25 ng/mL) or BMSCs (HS-5) for 48 h. * p <0.05 compared with the 0 μM group, & p <0.05. ( F ) The percentage of Annexin-V positive cells. * p <0.05 compared with the 0 μM group. ( G ) BMSCs, ARP1, or both ARP1 and BMSCs cells were treated with DCZ0814 for 48 h, and apoptosis was detected by Annexin-V/PI staining followed by flow cytometry. Green represents BMSCs HS-5 cells, and red represents ARP1 cells. In each case, data are presented as the mean ± SD of triplicate treatments. Abbreviations: MM, multiple myeoma; BM, bone marrow; CCK-8, cell counting kit-8; IL-6, interleukin-6; IGF-1, insulin-like growth factor-1; BMSC, bone marrow stromal cell.
    Figure Legend Snippet: DCZ0814 induced cytotoxicity in MM cells and overcome the protective effect of the BM microenvironment. ( A ) Molecular structure of DCZ0814. ( B ) MM cells treated with DCZ0814 for 48 h and cell viability determined by CCK-8 assay. ( C ) MM cells treated with DCZ0814 for the indicated time and cell viability determined by CCK-8 assay. ( D and E ) Cell viability of ARP1 and OCI-MY5 cells treated with DCZ0814 alone or in the presence of IL-6 (25 ng/mL), IGF-1 (25 ng/mL) or BMSCs (HS-5) for 48 h. * p <0.05 compared with the 0 μM group, & p <0.05. ( F ) The percentage of Annexin-V positive cells. * p <0.05 compared with the 0 μM group. ( G ) BMSCs, ARP1, or both ARP1 and BMSCs cells were treated with DCZ0814 for 48 h, and apoptosis was detected by Annexin-V/PI staining followed by flow cytometry. Green represents BMSCs HS-5 cells, and red represents ARP1 cells. In each case, data are presented as the mean ± SD of triplicate treatments. Abbreviations: MM, multiple myeoma; BM, bone marrow; CCK-8, cell counting kit-8; IL-6, interleukin-6; IGF-1, insulin-like growth factor-1; BMSC, bone marrow stromal cell.

    Techniques Used: CCK-8 Assay, Staining, Flow Cytometry, Cell Counting

    Related Articles

    CCK-8 Assay:

    Article Title: DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2
    Article Snippet: The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).. NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).. The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.

    Article Title: <p>DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2</p>
    Article Snippet: Cells and cell culture The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).Cells and cell culture The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).. NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).. The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.

    Staining:

    Article Title: DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2
    Article Snippet: The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).. NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).. The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.

    Article Title: <p>DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2</p>
    Article Snippet: Cells and cell culture The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).Cells and cell culture The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).. NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).. The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.

    Flow Cytometry:

    Article Title: DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2
    Article Snippet: The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).. NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).. The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.

    Article Title: <p>DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2</p>
    Article Snippet: Cells and cell culture The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).Cells and cell culture The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).. NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).. The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.

    Cell Counting:

    Article Title: DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2
    Article Snippet: The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).. NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).. The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.

    Article Title: <p>DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2</p>
    Article Snippet: Cells and cell culture The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).Cells and cell culture The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).. NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).. The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.

    Cytometry:

    Article Title: DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2
    Article Snippet: The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).. NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).. The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.

    Article Title: <p>DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2</p>
    Article Snippet: Cells and cell culture The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).Cells and cell culture The human MM cell lines ARP1, OCI-MY5, the bortezomib-sensitive MM cell line RPMI-8226 and the bortezomib-resistant cell line RPMI-8226/R5 were kindly provided by Fenghuang Zhan (Department of Internal Medicine, University of Iowa, Iowa City, IA, USA).. NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).. The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.The bortezomib-resistant cell line NCI-H929/bortezomib was cultured in the presence of 40 nM bortezomib.



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    ATCC bone marrow stroma cell bmsc line hs 5
    DCZ0814 induced cytotoxicity in MM cells and overcome the protective effect of the BM microenvironment. ( A ) Molecular structure of DCZ0814. ( B ) MM cells treated with DCZ0814 for 48 h and cell viability determined by CCK-8 assay. ( C ) MM cells treated with DCZ0814 for the indicated time and cell viability determined by CCK-8 assay. ( D and E ) Cell viability of ARP1 and OCI-MY5 cells treated with DCZ0814 alone or in the presence of IL-6 (25 ng/mL), IGF-1 (25 ng/mL) or BMSCs (HS-5) for 48 h. * p <0.05 compared with the 0 μM group, & p <0.05. ( F ) The percentage of Annexin-V positive cells. * p <0.05 compared with the 0 μM group. ( G ) BMSCs, ARP1, or both ARP1 and BMSCs cells were treated with DCZ0814 for 48 h, and apoptosis was detected by Annexin-V/PI staining followed by flow cytometry. Green represents BMSCs HS-5 cells, and red represents ARP1 cells. In each case, data are presented as the mean ± SD of triplicate treatments. Abbreviations: MM, multiple myeoma; BM, bone marrow; CCK-8, cell counting kit-8; IL-6, interleukin-6; IGF-1, insulin-like growth factor-1; <t>BMSC,</t> bone marrow stromal cell.
    Bone Marrow Stroma Cell Bmsc Line Hs 5, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bone+marrow+stroma+cell+bmsc+line+hs+5/HS-5/pmc06549748-13-5-16
    Average 97 stars, based on 1 article reviews
    bone marrow stroma cell bmsc line hs 5 - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

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    DCZ0814 induced cytotoxicity in MM cells and overcome the protective effect of the BM microenvironment. ( A ) Molecular structure of DCZ0814. ( B ) MM cells treated with DCZ0814 for 48 h and cell viability determined by CCK-8 assay. ( C ) MM cells treated with DCZ0814 for the indicated time and cell viability determined by CCK-8 assay. ( D and E ) Cell viability of ARP1 and OCI-MY5 cells treated with DCZ0814 alone or in the presence of IL-6 (25 ng/mL), IGF-1 (25 ng/mL) or BMSCs (HS-5) for 48 h. * p <0.05 compared with the 0 μM group, & p <0.05. ( F ) The percentage of Annexin-V positive cells. * p <0.05 compared with the 0 μM group. ( G ) BMSCs, ARP1, or both ARP1 and BMSCs cells were treated with DCZ0814 for 48 h, and apoptosis was detected by Annexin-V/PI staining followed by flow cytometry. Green represents BMSCs HS-5 cells, and red represents ARP1 cells. In each case, data are presented as the mean ± SD of triplicate treatments. Abbreviations: MM, multiple myeoma; BM, bone marrow; CCK-8, cell counting kit-8; IL-6, interleukin-6; IGF-1, insulin-like growth factor-1; BMSC, bone marrow stromal cell.

    Journal: Cancer Management and Research

    Article Title: DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2

    doi: 10.2147/CMAR.S194202

    Figure Lengend Snippet: DCZ0814 induced cytotoxicity in MM cells and overcome the protective effect of the BM microenvironment. ( A ) Molecular structure of DCZ0814. ( B ) MM cells treated with DCZ0814 for 48 h and cell viability determined by CCK-8 assay. ( C ) MM cells treated with DCZ0814 for the indicated time and cell viability determined by CCK-8 assay. ( D and E ) Cell viability of ARP1 and OCI-MY5 cells treated with DCZ0814 alone or in the presence of IL-6 (25 ng/mL), IGF-1 (25 ng/mL) or BMSCs (HS-5) for 48 h. * p <0.05 compared with the 0 μM group, & p <0.05. ( F ) The percentage of Annexin-V positive cells. * p <0.05 compared with the 0 μM group. ( G ) BMSCs, ARP1, or both ARP1 and BMSCs cells were treated with DCZ0814 for 48 h, and apoptosis was detected by Annexin-V/PI staining followed by flow cytometry. Green represents BMSCs HS-5 cells, and red represents ARP1 cells. In each case, data are presented as the mean ± SD of triplicate treatments. Abbreviations: MM, multiple myeoma; BM, bone marrow; CCK-8, cell counting kit-8; IL-6, interleukin-6; IGF-1, insulin-like growth factor-1; BMSC, bone marrow stromal cell.

    Article Snippet: NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: CCK-8 Assay, Staining, Flow Cytometry, Cell Counting